The first five protein preps were beautiful! I knew exactly where the protein eluted. I could see it. And then Wham! It has stopped working. I have no clue and I am absolutely going crazy trying to figure out what has happened.
The day starts at 7.30am in the morning when I open the lab. Very often it ends at 7.30pm when I pack up and leave. And after 12 hours if the protein purification does not work, all you feel like doing is wailing.
Bah!
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